A platinum(II) phenylphenanthroimidazole with an extended side-chain exhibits slow dissociation from a c-kit G-quadruplex motif
Katherine J. Castor, Zhaomin Liu, Johans Fakhoury, Mark A. Hancock, Anthony Mittermaier, Nicolas Moitessier, and Hanadi F. Sleiman
Chem. Eur. J., 2013, ,

A series of three platinum(II) phenanthroimidazoles each containing a protonable side-chain appended from the phenyl moiety through Copper(I)-catalyzed Azide-Alkyne Cycloaddition (CuAAC) were evaluated for their capacities to bind to human telomere, c-myc, and c-kit derived G-quadruplexes. We optimized the side-chain to enable a multivalent binding mode to G-quadruplex motifs, which would potentially result in selective targeting. Molecular modeling, high-throughput fluorescence intercalator displacement (HT-FID) assays, and surface plasmon resonance (SPR) studies demonstrate that complex 2 exhibits significantly slower dissociation rates compared to platinum phenanthroimidazoles without side-chains and other reported G-quadruplex binders. Complex 2 showed little cytotoxicity in HeLa and A172 cancer cells lines, consistent with the fact that it does not follow a telomere-targeting pathway. Preliminary mRNA analysis shows that 2 specifically interacts with the ¬c-kit promoter region. Overall, this study validates 2 as a useful molecular probe for c-kit related cancer pathways.

Site-specific positioning of dendritic alkyl chains on NA cages enables their geometry dependent self-assembly
T. Edwardson, C.K. McLaughlin, K. Carneiro, C. Serpell, H.F. Sleiman
Nature Chem, 2013, ,

Nature uses a combination of non-covalent interactions to create a hierarchy of complex systems from simple building blocks. One example is the selective association of the hydrophobic side chains that are a strong determinant of protein organization. Here, we report a parallel mode of assembly in DNA nanotechnology. Dendritic alkyl-DNA conjugates are hybridized to the edges of a DNA cube. When four amphiphiles are on one face, the hydrophobic residues of two neighbouring cubes engage in an intermolecular ‘handshake’, resulting in a dimer. When there are eight amphiphiles (four on the top and bottom cube faces, respectively), they engage in an intramolecular ‘handshake’ inside the cube. This forms the first example of a monodisperse micelle within a DNA nanostructure that encapsulates small molecules and releases them by DNA recognition. Creating a three-dimensional pattern of hydrophobic patches, like side chains in proteins, can result in specific, directed association of hydrophobic domains with orthogonal interactions to DNA base-pairing.
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Intercalators as Molecular Chaperones in DNA Self-Assembly
A. Greschner, K. Bujold, H.F. Sleiman
J. Am. Chem. Soc., 2013, 135, 11283

DNA intercalation has found many diagnostic and therapeutic applications. Here, we propose the use of simple DNA intercalators, such as ethidium bromide, as tools to facilitate the error-free self-assembly of DNA nanostructures. We show that ethidium bromide can influence DNA self-assembly, decrease the formation of oligomeric side products, and cause libraries of multiple equilibrating structures to converge into a single product. Using a variety of 2D- and 3D-DNA systems, we demonstrate that intercalators present a powerful alternative for the adjustment of strand-end alignment, favor the formation of fully duplexed “closed” structures, and create an environment where the smallest, most stable structure is formed. A new 3D-DNA motif, the ninja star, was self-assembled in quantitative yield with this method. Moreover, ethidium bromide can be readily removed using isoamyl alcohol extractions combined with intercalator-specific spin columns, thereby yielding the desired ready-to-use DNA structure.
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Gold Nanoparticle 3D-DNA Building Blocks: High Purity Preparation and Use for Modular Access to Nanoparticle Assemblies
K.L. Lau, G. Hamblin, H.F. Sleiman
Small, 2013, ,

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A Simiple Design for DNA Nanotubes from a Minimal Set of Unmodified Strands: Rapid, Room Temperature Assembly and Readily Tunable Structure
G. Hamblin, A. Hariri, K. Carnerio, K. Lau, G. Cosa, H.F. Sleiman
ACS Nano, 2013, 7, 3022

DNA nanotubes have great potential as nanoscale scaffolds for the organization of materials and the templation of nanowires and as drug delivery vehicles. Current methods for making DNA nanotubes either rely on a tile-based step-growth polymerization mechanism or use a large number of component strands and long annealing times. Step-growth polymerization gives little control over length, is sensitive to stoichiometry, and is slow to generate long products. Here, we present a design strategy for DNA nanotubes that uses an alternative, more controlled growth mechanism, while using just five unmodified component strands and a long enzymatically produced backbone. These tubes form rapidly at room temperature and have numerous, orthogonal sites available for the programmable incorporation of arrays of cargo along their length. As a proof-of-concept, cyanine dyes were organized into two distinct patterns by inclusion into these DNA nanotubes.
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DNA Nanostructure serum stability: greater than the sum of its parts
J. Conway, C.K. McLaughlin, K. Castor, H.F. Sleiman
Chem. Commun., 2013, 49, 1172

Simple chemical modifications to oligonucleotide ends with hexaethylene glycol and hexanediol are shown to significantly increase nuclease resistance under serum conditions. The modified oligonucleotides were used to construct DNA prismatic cages in a single step and in quantitative yield. These cages further stabilize their strands towards nucleases, with lifetimes of 62 hours in serum. The cages contain a large number of single-stranded regions for functionalization, illustrating their versatility for biological applications.
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Long-Range Assembly of DNA into nanofibers and organized Networks
K. Carneiro, N. Avakyan, H.F. Sleiman
WIRE Nanomedicine and Nanobiotechnology, 2013, 5, 266

Long-range assembly of DNA currently comprises both top-down and bottom-up methods. The top-down techniques consist of physical alignment of DNA and lithographic patterning to organize DNA on surfaces. The bottom-up approaches include lipid and polymer DNA co-assembly, the self-assembly of DNA amphiphiles, and the remarkably specific and versatile methods of DNA nanotechnology. DNA-based materials possess unprecedented molecular control and may offer innovative solutions in the fields of nanotechnology, sensing, nanomedicine, as well as optical and electronic devices. To realize the potential of these materials, a number of hurdles must be addressed. Bridging the gap between top-down fabrication and bottom-up assembly is of critical importance to the successful development of functional DNA-based technology. A profound understanding of both regimes is necessary to achieve this goal. WIREs Nanomed Nanobiotechnol 2013 5 266 285. doi: 10.1002/wnan.121
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Stimuli-responsive organization of block copolymers on DNA nanotubes
K. Carneiro, G. Hamblin, H.F. Sleiman
Chemical Science, 2013, 3, 1980

DNA nanotubes are an attractive class of materials that can be assembled with precise control over their size, shape, length and porosity, and can encapsulate and release materials in response to specific added molecules. In parallel, block copolymer assemblies can provide biocompatibility, stability, nuclease resistance, the ability to encapsulate small molecules, long-range assembly and numerous additional functionalities that can be tuned with subtle chemical modifications. Herein, we describe a new class of hybrid materials in which block copolymer assemblies are sequence-specifically and longitudinally positioned on robust DNA nanotubes constructed using rolling circle amplification. These materials are dynamic, allowing the polymer structures to be cleanly removed from the DNA nanotubes only when a specific DNA sequence is added, and creating a single-stranded form of these nanotubes. Specifically, we first describe the use of rolling circle amplification to create a long DNA strand containing a repeat sequence. This is used as a guide strand in a new method to construct robust nanotubes with a non-nicked backbone. We then synthesize polymer DNA conjugates through on-column functionalization of a DNA strand with a polymer. These conjugates self-assemble into spherical aggregates, which then position themselves onto the DNA nanotubes using sequence-specific hybridization, creating a 'striped' structure. The polymer aggregates can be cleanly lifted off the nanotubes using a strand displacement strategy, thus uncapping these DNA nanotubes. We also show that this longitudinal alignment of polymer aggregates on DNA nanotubes is general for a variety of DNA block copolymers.
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  • + A. Greschner, V. Toader, H.F. Sleiman, 'The role of Organic Linkers in Directing DNA Self-Assembly and Significantly Stabilizing DNA Duplexes', J. Am. Chem. Soc., 2012, 134, 14382
    We show a simple method to control both the stability and the self-assembly behavior of DNA structures. By connecting two adjacent duplexes with small synthetic linkers, factors such as linker rigidity and DNA strand orientation can increase the thermal denaturation temperature of 17 base-pair duplexes by up to 10 �C, and significantly increase the cooperativity of melting of the two duplexes. The same DNA sequence can thus be tuned to melt at vastly different temperatures by selecting the linker structure and DNA-to-linker connectivity. In addition, a small rigid m-triphenylene linker directly affects the self-assembly product distribution. With this linker, changes in the orientation of the linked strands (e.g., 5?3? vs 3?3?) can lead to dramatic changes in the self-assembly behavior, from the formation of cyclic dimer and tetramer to higher-order oligomers. These variations can be readily predicted using a simple strand-end alignment model.
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  • + C.K. McLaughlin, G.D. Hamblin, K. Haenni, J.W. Conway, M.K. Nayak, K.M.M. Carneiro, H.S. Bazzi, H.F. Sleiman, 'Three-Dimensional Organization of Block Copolymers on DNA-minimal Scaffolds', J. Am Chem. Soc., 2012, 134, 4280
    Here, we introduce a 3D-DNA construction method that assembles a minimum number of DNA strands in quantitative yield, to give a scaffold with a large number of single-stranded arms. This DNA frame is used as a core structure to organize other functional materials in 3D as the shell. We use the ring-opening metathesis polymerization (ROMP) to generate block copolymers that are covalently attached to DNA strands. Site-specific hybridization of these DNA-polymer chains on the single-stranded arms of the 3D-DNA scaffold gives efficient access to DNA-block copolymer cages. These biohybrid cages possess polymer chains that are programmably positioned in three dimensions on a DNA core and display increased nuclease resistance as compared to unfunctionalized DNA cages.
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  • + G.D. Hamblin, K.M.M. Carneiro, J. Fakhoury, K. Bujold, H.F. Sleiman, 'Rolling Circle Amplification-Templated DNA nanotubes Show Increased Stability and Cell Penetration Ability', J. Am. Chem. Soc., 2012, 134, 2888
    DNA nanotubes hold promise as scaffolds for protein organization, as templates of nanowires and photonic systems, and as drug delivery vehicles. We present a new DNA-economic strategy for the construction of DNA nanotubes with a backbone produced by rolling circle amplification (RCA), which results in increased stability and templated length. These nanotubes are more resistant to nuclease degradation, capable of entering human cervical cancer (HeLa) cells with significantly increased uptake over double-stranded DNA, and are amenable to encapsulation and release behavior. As such, they represent a potentially unique platform for the development of cell probes, drug delivery, and imaging tools.
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  • + Castor, K.J., Mancini, J., Fakhoury, J., Weill, N., Kieltyka, R., Englebienne, P., Avakyan, N., Mittermaier, A., Autexier, C. , Moitessier, N. , Sleiman, H.F. 'Platinum(II) phenanthroimidazoles for targeting telomeric G-quadruplexes', ChemMedChem, 2012, 7, 85
    A rationally designed progression of phenanthroimidazole platinum(II) complexes were examined for their ability to target telomere-derived intramolecular G-quadruplex DNA. Through the use of circular dichroism, fluorescence displacement assays, and molecular modeling we show that these complexes template and stabilize G-quadruplexes from sequences based on the human telomeric repeat (TTAGGG)n. The greatest stabilization was observed for the p-chlorophenyl derivative 6 (G4DC50=0.31um). We also show that the G-quadruplex binding complexes are able to inhibit telomerase activity through a modified telomerase repeat amplification protocol (TRAP-LIG assay). Preliminary cell studies show that complex 6 is preferentially cytotoxic toward cancer over normal cell lines, indicating its potential use in cancer therapy.
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  • + K. Metera, K. Haenni, M. Nayak, H.S. Bazzi, H.F. Sleiman, 'Luminescent Iridium(III)-Containing Block Copolymers: Self-Assembly into Biotin-abeled Micelles for Biodetection Assays', ACS Macro Letters, 2012, 1, 954
    Luminescent polymers containing Ir(ppy)2(bpy) PF6 complexes, biocompatible poly(ethylene glycol) (PEG) chains, and biotin moieties were synthesized via ring-opening metathesis polymerization (ROMP). Their self-assembly in water into micelles resulted in an increased quantum yield compared to open polymer chains in acetonitrile, which is likely due to core rigidity and desolvation. Streptavidin coated magnetic beads were employed to analyze the binding ability of these micelles. The positioning of the molecular recognition moiety biotin within the polymer chain had a very significant effect on the availability of biotin on the micelle surface and the ability of the micelles to bind to streptavidin. Simply attaching biotin to the end of the ROMP polymer yielded micelles in which the biotin units were shielded by the PEG chains, whereas the synthesis of a new ROMP monomer containing biotin at the end of the PEG chains resulted in improved surface availability of the biotin group. Preliminary experiments in which streptavidin was microcontact-printed onto functionalized glass coverslips also indicated specific binding between the micelles and streptavidin and further demonstrated the potential of these micelle systems to function as luminescent probes in solid-phase biodetection assays.
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  • 2011
  • + C.K. McLaughlin, G. Hamblin, H.F. Sleiman, 'Supramolecular DNA Assembly', Chem. Soc. Rev, 2011, ,
    The powerful self-assembly features of DNA make it a unique template to finely organize and control matter on the nanometre scale. While DNA alone offers a high degree of fidelity in its self-assembly, a new area of research termed �supramolecular DNA assembly� has recently emerged. This field combines DNA building blocks with synthetic organic, inorganic and polymeric structures. It thus brings together the toolbox of supramolecular chemistry with the predictable and programmable nature of DNA. The result of this molecular partnership is a variety of hybrid architectures, that expand DNA assembly beyond the boundaries of Watson�Crick base pairing into new structural and functional properties. In this tutorial review we outline this emerging field of study, and describe recent research aiming to synergistically combine the properties inherent to DNA with those of a number of supramolecular scaffolds. This ultimately creates structures with numerous potential applications in materials science, catalysis and medicine.
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  • + C.K. McLaughlin, G.D. Hamblin, F.A. Aldaye, H. Yang, H.F. Sleiman, 'A Facile, Modular and High Yield Method to Assemble Three-Dimensional DNA Structures', Chem. Comm., 2011, 47, 8925
    We describe a rapid and quantitative method to generate DNA cages of deliberately designed geometry from readily available starting strands. Balancing the incorporation of sequence uniqueness and symmetry in a face-centered approach to 3D construction can result in triangular (TP), rectangular (RP), and pentagonal prisms (PP) without compromising the potential for nanostructure addressability.
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  • + H. Yang, F. Altvater, A.D. de Bruijn, C.K. McLaughlin, P.K. Lo, H.F. Sleiman, 'Chiral four-arm metal-DNA junctions and metalated DNA Nanotubes', Angew. Chem., 2011, 50, 4620
    Chiral DNA junctions with a transition metal at the branch point and four different DNA single-stranded arms were assembled by a combination of DNA templating and metal coordination in an approach that enabled chirality transfer from DNA to the metal center (see picture). The intrinsic chirality of the junctions and their information density make them effective building blocks for 3D metal�DNA
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  • 2010
  • + P.K. Lo, F. Altvater, H.F. Sleiman, 'Templated Synthesis of DNA Nanotubes with controlled Predetermined Lengths', J. Am. Chem. Soc., 2010, 132, 10212
    We report a DNA-templated approach to construct nanotubes with controlled lengths and narrow molecular weight distribution, allowing the deliberate variation of this length. This approach relies on the facile and modular assembly of a DNA guide strand of precise length that contains single-stranded gaps repeating at every 50 nm. This is followed by positioning triangular DNA �rungs� on each of these single-stranded gaps and adding identical linking strands to the two other sides of the triangles to close the DNA nanotubes. The length of the guide strand can be deliberately changed. We show the use of this approach to produce nanotubes with lengths of 1 ?m or 500 nm and narrow length distributions. This is in contrast to nontemplated approaches, which lead to long and polydisperse nanotubes. We also demonstrate the encapsulation of 20 nm gold nanoparticles within these well-defined nanotubes to form finite lines of gold nanoparticles with longitudinal plasmon coupling, with a number of potential nanophotonic applications. This guiding strand approach is a useful tool in the creation of DNA nanostructures, in this case allowing the use of a simple template generated by a minimal number of DNA strands to program the length and molecular weight distribution of assemblies, as well as to organize any number of DNA-labeled nano-objects into finite structures.
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  • + P.K. Lo, K. Metera, H.F. Sleiman, 'Self-Assembly of Three-Dimensional DNA Nanostructures and Ptoential Biological Applications', Curr. Opinion in Chemical Biology, 2010, 14, 597
    A current challenge in nanoscience is to achieve controlled organization in three-dimensions, to provide tools for biophysics, molecular sensors, enzymatic cascades, drug delivery, tissue engineering, and device fabrication. DNA displays some of the most predictable and programmable interactions of any molecule, natural or synthetic. As a result, 3D-DNA nanostructures have emerged as promising tools for biology and materials science. In this review, strategies for 3D-DNA assembly are discussed. DNA cages, nanotubes, dendritic networks, and crystals are formed, with deliberate variation of their size, shape, persistence length, and porosities. They can exhibit dynamic character, allowing their selective switching with external stimuli. They can encapsulate and position materials into arbitrarily designed patterns, and show promise for numerous biological and materials applications.
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  • + P.K. Lo, P. Karam, F. Aldaye, G. Hamblin, G. Cosa, H.F. Sleiman, 'Loading and Selective Release of Cargo in DNA Nanotubes with Longitudinal Variation', Nature Chemistry, 2010, 2, 319
    Nanotubes hold promise for a number of biological and materials applications because of their high aspect ratio and encapsulation potential. A particularly attractive goal is to access nanotubes that exert well-de?ned control over their cargo, such as selective encapsulation, precise positioning of the guests along the nanotube length and triggered release of this cargo in response to speci?c external stimuli. Here, we report the construction of DNA nanotubes with longitudinal variation and alternating larger and smaller capsules along the tube length. Size-selective encapsulation of gold nanoparticles into the large capsules of these tubes leads to �nanopeapod� particle lines with positioning of the particles 65 nm apart. These nanotubes can then be opened when speci?c DNA strands are added to release their particle cargo spontaneously. This approach could lead to new applications of self-assembled nanotubes, such as in the precise organization of one-dimensional nanomaterials, gene-triggered selective delivery of drugs and biological sensing.
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  • + Y. Wen, C.K. McLaughlin, P.K. Lo, H. Yang, H.F. Sleiman, 'Stable Gold nanoparticle Conjugation to Internal DNA Positions: Facile Generation of Discrete Gold Nanoparticle DNA assemblies', Bioconj. Chem., 2010, 21, 1413
    A straightforward strategy to stably anchor one or more gold nanoparticles (AuNPs) at both the internal and terminal positions of single-stranded DNA is presented. Discrete DNA?AuNP conjugates are isolated using a noncovalent extension strand strategy, that helps to resolve prepared species by agarose gel electrophoresis (AGE). These are used to assemble well-defined AuNP squares and rectangles. Two complementary bis-AuNP-labeled DNA conjugates are then prepared. One of these places two smaller (5 nm) AuNPs at defined internal positions within a DNA strand, and the other places two larger (13 nm) AuNPs at each of its terminal positions. We show the self-assembly of these bis-AuNP conjugates into a tapered tetrameric gold nanoparticle 'antenna' structures of direct relevance to engineered �hot spots� and surface enhanced Raman scattering (SERS) substrates.
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  • + N.B. Sankaran, A. Rys, R. Nassif, M.K. Nayak, K. Metera, B. Chen, H.S. Bazzi, h.F. Sleiman, 'Ring-Opening Metathesis Polymers for Biodetection and Signal Amplification: Synthesis and Slef-Assembly', Macromolecules, 2010, 43, 5530
    The ring-opening metathesis polymerization (ROMP) was used to develop a new class of block copolymers toward biological detection with signal amplification. For this, three classes of ROMP monomers were synthesized: (i) luminescent and electrochemiluminescent transition-metal-containing monomers, with ruthenium, osmium, and iridium bipyridine units, (ii) biologically compatible monomers and macromonomers containing oligothylene glycol units, and (iii) bioconjugatable monomers as well as monomers containing the biorecognition unit biotin. ROMP was used to efficiently combine these monomers into amphiphilic di- and triblock copolymers. Self-assembly of these block copolymers in aqueous media generates micellar spherical assemblies, which contain a large number of luminescent transition-metal centers in their core, a biocompatible and biologically inert protecting shell, and biological recognition units or bioconjugatable groups on their periphery. These micelles can act as luminescent markers for biological molecules with potential for signal amplification. In addition, the monomers and polymers reported here can serve as useful biologically enabled building blocks for a number of applications, including drug delivery and tissue engineering.
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  • + H. Yang, K. Metera, H.F. Sleiman, 'DNA modified with metal complexes: applications in the construction of higher-order metal-DNA nanostructures', Coord. Chem. Rev., 2010, 254, 2403
    DNA has recently emerged as a useful building block for higher order nanostructures, such as extended two-dimensional surfaces and discrete two- and three-dimensional structures. Transition metal complexes can introduce functionality to these otherwise passive nanostructures. This review examines the synthetic strategies used to introduce metals in a site-specific manner to DNA: either by attaching preformed metal complexes to DNA, or by metal coordination to unmodified or modified DNA. The applications of metal�DNA complexes in building higher order nanostructures and the utility of attaching luminescent or electrochemical labels are discussed.

    Keywords DNA; Self-assembly; Transition metal; Nanostructures

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  • 2009
  • + K. Carneiro, H.F. Sleiman, 'Long-Rane Assembly of DNA into Rigid Nanofibres and Highly Ordered Network using a Block Copolymer Approach', J. Am. Chem. Soc., 2009, 132, 679
  • + H. Yang, A. Rys, C.M. McLaughlin, H.F. Sleiman, 'Templated Ligand Environments for the Selective Incorporation of Different metals into DNA', Angew. Chem., 2009, 48, 9919
  • + H. Yang, C. McLaughlin, G. Hamblin, F. Aldaye, A. Rys, I. Rouiller, H.F. Sleiman, 'Metal Nucleic Acid Cages', Nature Chemistry, 2009, 1, 390
  • + F.A. Aldaye, P.K. Lo, P. Karam, G. Cosa, H.F. Sleiman, 'Modular Construction of DNA nanotubes with tunable geometry and single- or doulbe-stranded character', Nature Nanotechnology, 2009, 4, 349
  • + P.K. Lo, H.F. Sleiman, 'Nucleobase-Templated Polymerization: Copying the Chain Lenght and Polydispersity of Living Polymers into Conjugated Polymers', J. Am. Chem. Soc., 2009, 131, 4182
  • + S.E. Pierce, R. Kieltyka, H.F. Sleiman, J.S. Brodbelt, 'Evaluation of Binding Selectivities and Affinities of Platinum-Based Quadruplex Interactive Complexes by Electrospray Ionization mass Spectroscopy', Biopolymers, 2009, 91, 233
  • + I. Ozturk, S.K. Hadjikakou, N. Hadjiliadis, N. Kourkoumelis, M. Kubicki, A.T. Tasiopoulos, H.F. Sleiman, M.M. Barsan, I.S. Butler, J. Balzarini, 'New Antimoney(III) Bromide Complexes with Thioamides: Synthesis, Characterization, and Cytostatic Properties', Inorg. Chem., 2009, 48, 1811
  • 2008
  • + F.A. Aldaye, A. Palmer, H.F. Sleiman, 'Assembling Materials with DNA as the Guide', Science, 2008, 321, 1795
  • + R. Kieltyka, P. Englebienne, J. Fakhoury, C. Autexier, N. Moitessier, H.F. Sleiman, 'A Platinum Supramolecular Square as an Effective G-Quadruplex Binder and Telomerase Inhibitor', J. Am. Chem. Soc., 2008, 130, 10040
  • + P.K. Lo, H.F. Sleiman, 'Synthesis and Molecular Recognition properties of DNA-Mimetic Conjugated Polymer', Macromolecules, 2008, 41, 5590
  • + H. Yang, H.F. Sleiman, 'Templated Synthesis of Highly Stable, Electroactive and Dynamic Metal-DNA Branched Junctions', Angew. Chemie, 2008, 47, 2443
  • 2007
  • + F. Aldaye, H.F. Sleiman, 'Modular Generation of Structurally Switchable 3D- DNA Assemblies', J. Am. Chem. Soc., 2007, , 13376
  • + R. Kieltyka, J. Fakhoury, N. Moitessier, H.F. Sleiman, 'Platinum Phenanthroimidazole Complexes as G-Quadruplex DNA Selective Binders', Chemistry, a European Journal, 2007, 14, 1145
  • + F. Aldaye, H.F. Sleiman, 'Guest-Mediated Access to a Single DNA Nanostructure from a Library of Multiple Assemblies', J. Am. Chem. Soc., 2007, 129, 10070
  • + F. Aldaye, H.F. Sleiman, 'Dynamic DNA Templates for Gold Nanoparticle Discrete Structures: Control of Geometry, Particle Identity, Write/Erase and Strucutreal Switching', J. Am. Chem. Soc, 2007, 129, 4130
  • + K. Metera, H.F. Sleiman, 'Luminescent Vesicles, Tubules, Bowls and Star Micelles from ruthenium Bipyridine Block Copolymers', Macromolecules, 2007, 40, 3733
  • + M. Slim, N. Durisic, P. Grutter, H.F. Sleiman, 'DNA-Protein Non-Covalent Crosslinking: Ruthenium Dipyridophenazine Biotin Complex for the Assembly of Proteins and Gold Nanoparticles on DNA Templates', ChemBioChem, 2007, 8, 804
  • + Y. Ishihara, H.S. Bazzi, V. Toader, F. Godin, H.F. Sleiman, 'Molecule-Resposive Block Copolymer Micelles', Chemistry, a European Journal, 2007, 13, 4560
  • + F. Rakotondradany, H.F. Sleiman, M.A. Whitehead, 'Theoretical study on self-assembled H-bonded azodibenzoic acid tapes and rosettes', J. Mol. Struct. Theochem., 2007, 806, 39
  • 2006
  • + F. Aldaye, H.F. Sleiman, 'Sequential Self-Assembly of a DNA Hexagon as a Template for the Orgaization of Gold Nanoparticles', Angew. Chem. Int. Ed. Engl, 2006, 45, 2204
  • 2005
  • + B. Chen, H.F. Sleiman, 'Biotin-Terminated Ruthenium Bipyridine ROMP Polymers: Synthesis an Self-Assembly with Streptavidin', Macromolecules, 2005, 38, 1084
  • + F. Rakotondradany, A. Palmer, H.F. Sleiman, 'Hydrogen-Bond Self-Assembly of DNA Analogues into a Hexameric Rosette', Chem. Commun, 2005, , 5441
  • 2004
  • + D. Mitra, N. Di Cesare, H.F. Sleiman, 'Self-Assembly of Cyclic Metal-DNA nanostructures Using Ruthenium Bipyridine Branched Oligonucleotides', Angew. Chem. , 2004, 43, 5804
  • + M. Slim, H.F. Sleiman, 'Ruthenium(II) Phenanthroline-Biotin Complexes: Synthesis and Luminescence Enhancement upon Biding to avidin', Bioconjugate Chem., 2004, 15, 949
  • + B. Chen, H.F. Sleiman, 'Ruthenium Bipyridine-Containing Polymers and Block Copolymers via Ring-Opening Metathesis Polymerization', Macromolecules, 2004, 37, 5866
  • + A. Rezvani, H.S. Bazzi, B. Chen, F. Rakotondradany, H.F. Sleiman, 'Ruthenium(II) Dipyridoquinoxaline-orbornene: Synthesi, Properties, Crystal Structure and Use as ROMP monomer', Inorg. Chem., 2004, 43, 5112
  • 2003
  • + H.S. Bazzi, J. Bouffard, H.F. Sleiman, 'Self-Complementary ABC Triblock Copolymers via Ring-Opening Metathesis Polymerization', Macromolecules, 2003, 36, 7899
  • + F. Rakotondradany, M.A. Whitehead, H.F. Sleiman, 'Photoresponsive Supramolecular Systems: Self-Assembly of Azobenzoic Acid Linear Tapes and Cyclic Tetramers', Chemistry, a European Journal, 2003, 9, 4771
  • 2002
  • + H.S. Bazzi, H.F. Sleiman, 'Adenine-Containing Block Copolymers via Ring Opening Metathesis Polymerization: Sytnehsis and Self-Assembly into Rod Morphologies', Macromolecules, 2002, , 9617
  • + J. Dalphond, H. Bazzi, K. Kahrim, H.F. Sleiman, 'Synthesis and Self-Assembly of Polymers containing Dicarboximide Groups by Living Ring-Opneing Metathesis Polymerization', Macromolecular Chem. Phys, 2002, 203, 1988
  • + H. Bazzi, H.F. Sleiman, 'Synthesis and Self Assembly of Conjugated Polymer Precursors Containing Dichlorocarbonate Groups by Living Ring-Opnening Metathesis Polymerization', Macromolecules, 2002, 35, 624
  • 2001
  • + I.Varga-Baca, D. Mitra, H. Zuyniak, J. Banerjee, H.F. Sleiman, 'Solid-Phase Synthesis of Transition Metal-Linked Branched Oligonucleotides', Angew. Chem. Int. Ed. Engl, 2001, 40, 4629
  • 2000 and earlier
  • + P.N.W. Baxter, H.F. Sleiman, J.M. Lehn, K. Rissanen, 'Multicomponent Self-Assembly of [3]-Pseudorotaxanes', Angew. Chem. Int. Ed. Engl., 1997, 36, 1294
    plaatje en abstract en download
  • + H.F. Sleiman, P.N.W. Baxter, J.M. Lehn, . Airola, K. Rissanen, 'Multicomponent Self-Assembly: The generation of Rigid-Rack multimetallic Pseudorotaxanes', Inorg Chem. , 1997, 36, 4734
    plaatje en abstract en download
  • + H.F. Sleiman, P.N.W. Baxter, J.M. Lehn, K. Rissanen, 'Self-Assembly of Rigid-Rack Multimetallic Complexes of Rotaxane Type', Chem. Commun., 1995, , 715
    plaatje en abstract en download
  • + B.A. Arndtsen, H.F. Sleiman, L. McElwee-White, A. Rheingold, 'Elecrophilic Reactions of Zero-Valent Tungsten Nitrene and Hydrazido Complexes with Phosphines. Synthesis and structure of cis-(CO)4WP(Ph)2CH2P(Ph)2NNNMe2', Organometallics, 1993, 12, 2440
    plaatje en abstract en download
  • + C.T. Maxey, H.F. Sleiman, L. McElwee-White, 'Metathesis and Diaziridination Reactions of (CO)5W=C(OMe)(p-XC6H4) with cis-Azobenzene. Electronic and Solvent Effects.', J. Am. Chem. Soc., 1992, 114, 5153
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  • + B.A. Arndtsen, H.F. Sleiman, A.K. Chang, L. McElwee-White, 'Evidence for Ambiphilic Behavior in (CO)5W=NPh. Conversion of Carbony Compounds to N-Phenyl Imines via Metathesis', J. Am. Chem. Soc., 1991, 113, 4871
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  • + H.F. Sleiman, B.A. Arndtsen, L. McElwee-White, 'Direct Observation of the Low-Valent Hydrazido Complex (CO)5W=NNMe2, a nitrene Analogue of the Heteroatom-Stabilized Fischer Carbenes', Organometallics, 1991, 10, 541
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  • + H.F Sleiman, S. Mercer, L. McElwee-White, 'Trapping of the Low-Valent Nitrene Complex (CO)5W=NPh with PPh3. Formation of the Phenylnitrene Transfer Product PhN=PPh3', J. Am. Chem. Soc., 1989, 111, 8007
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  • + H.F. Sleiman, L. McElwee-White, 'Photochemical Azo Metathesis by (CO)5W=C(OCH3)(CH3). Isolation of a Zwitterionic Intermediate', J. Am. Chem. Soc., 1988, 110, 8700
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